How To Calculate Depth Of Field Formula. The default is to say that features smaller than 0.01 inches are unnecessary, when. For example between 250 to 2500nm in aluminium, is there a formula or excel table that we can calculate for all metal.

ānā is the refractive index of the material between the objective and the sample. The resulting depth of field starts from halfway of the hyperfocal distance and extends to infinity. It is generally assumed that problems involving depth of focus only occur with large sensors.